The procedure for MRSA diagnostic
This diagnostic protocol is a new and clinically
validated procedure that consists of a direct
one-step enrichment of MRSA present in either nasal
or inguinal swabs, followed by DNA extraction of
immunocaptured bacteria and their identification by
multiplex sequence amplification using real-time
quantitative PCR.
This protocol will be implemented with a simple
“hands-off” system based on: (1) novel strategies
for the integration of full operations required for
the entire nucleic acid analysis chain in a
microfluidic platform, and (2) advanced
microfluidic magnetic nanoparticles manipulation
technology allowing efficient capture and
extraction of target bacteria and nucleic acids.
The separate steps of sample preparation, signal
amplification by multiplex PCR, and simultaneous
detection of multiple genes, will be performed as
one single step using a ready-to-use disposable
fluidic chip.
Turning a new diagnostic protocol into a
one-step procedure
Microfluidics chip allowing the integration of 4
assays in parallel,
Schematic diagram describing the platform for
MRSA
diagnostics
Project Objectives